In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings.
Símboli N, Takiff H, McNerney R, López B, Martin A, Palomino JC, Barrera L, Ritacco V.
INEI-ANLIS "Carlos G. Malbrán," Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.
An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.
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PMID: 15616326 [PubMed - indexed for MEDLINE]PMCID: PMC538913