Table 1

Summary of the gene regions detected in the patients isolates from ascitic fluid and blood culture compared to the 569B reference organism and serogroup O21 609-68 and 418-03 strains kindly provided by the National Institute of Infectious Diseases, Tokyo, Japan.

Notes. Amplification by was done in a 25 μl reaction mixture containing 3 μl of template DNA, 200 μM of each dNTP, 1X reaction buffer, 1.25 units of Taq polymerase (TaKaRa Ex-Taq), and 0.5 μM of each primers. The initial amplification conditions were one cycle at 95°C for 7 minutes, 35 cycles at 95°C for 30 seconds. 55°C for 30 seconds, and 72°C for 30 seconds; and elongation step of 72°C for 5 minutes. Nested PCR was performed with the same method except that the annealing temperature was 60°C and the template was 2 μl of the first PCR product. Electrophoresis was performed on a 1.8% agarose gel.

Phetsouvanh et al. Annals of Clinical Microbiology and Antimicrobials 2008 7:10   doi:10.1186/1476-0711-7-10