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Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori

Shaik Mahaboob Ali123, Aleem A Khan24, Irshad Ahmed25, M Musaddiq5, Khaja S Ahmed2, H Polasa1, L Venkateswar Rao1, Chittoor M Habibullah24, Leonardo A Sechi46 and Niyaz Ahmed34*

Author Affiliations

1 Department of Microbiology, Osmania University, Hyderabad – 500 007, India

2 Centre for Liver Research and Diagnostics, Deccan Medical College & Allied Hospitals, Kanchanbagh, Hyderabad – 500 058, India

3 Pathogen Evolution Group, Center for DNA Finger printing and Diagnostics, Nacharam, Hyderabad- 500 076, India

4 ISOGEM Collaborative Network on Genetics of Helicobacters, The International Society for Genomic and Evolutionary Microbiology, Sassari, Italy

5 Department of Microbiology, Shri Shivaji College, Akola, India

6 Dipartimento di Scienze Biomediche, Sezione Microbiologia sperimentale e clinica, Facoltà di Medicina, Universiti degli studi de Sassari, viale S. Pietro, 07100 Sassari, Italy

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Annals of Clinical Microbiology and Antimicrobials 2005, 4:20  doi:10.1186/1476-0711-4-20

Published: 21 December 2005

Abstract

Background

Eradication of Helicobacter pylori is an important objective in overcoming gastric diseases. Many regimens are currently available but none of them could achieve 100% success in eradication. Eugenol and cinnamaldehyde that are commonly used in various food preparations are known to possess antimicrobial activity against a wide spectrum of bacteria.

Aim

The present study was performed to assess the in vitro effects of eugenol and cinnamaldehyde against indigenous and standard H. pylori strains, their minimum inhibitory concentrations (MICs) and time course lethal effects at various pH.

Methods

A total of 31 strains (29 indigenous and one standard strain of H. pylori ATCC 26695, one strain of E. coli NCIM 2089) were screened. Agar dilution method was used for the determination of drug sensitivity patterns of isolates to the commonly used antibiotics and broth dilution method for the test compounds.

Results

Eugenol and cinnamaldehyde inhibited the growth of all the 30 H. pylori strains tested, at a concentration of 2 μg/ml, in the 9th and 12th hours of incubation respectively. At acidic pH, increased activity was observed for both the compounds. Furthermore, the organism did not develop any resistance towards these compounds even after 10 passages grown at sub-inhibitory concentrations.

Conclusion

These results indicate that the two bioactive compounds we tested may prevent H. pylori growth in vitro, without acquiring any resistance.