Anti-nociceptive properties in rodents and the possibility of using polyphenol-rich fractions from sida urens L. (Malvaceae) against of dental caries bacteria
1 Unit of Formation in Sciences Applied and Technological (UFR/SAT) and Institute of Sciences of the Environment and the Rural Development (ISEDR), Polytechnic University of Dédougou, Dédougou, Burkina Faso
2 Laboratory of Plant Ecology and Biology, University of Ouagadougou, 09 PO Box: 848, Ouagadougou 09, Burkina Faso
3 Laboratory of Animal Physiology, University of Ouagadougou, 09 PO Box: 848, Ouagadougou 09, Burkina Faso
4 Laboratory of Food Biochemistry, Enzymology, Biotechnology and Bioinformatic, University of Ouagadougou, Ouagadougou, Burkina Faso
5 Laboratory of Biochemistry and Applied Chemistry, University of Ouagadougou, 09 PO Box: 848, Ouagadougou 09, Burkina Faso
6 Department of Plant Physiolgy and Ecology, Taras Shevchenko National University of Kyiv, Volodymyrs’ka St. 64, 01601, Kyiv, Ukraine
7 Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976, Nitra, Slovak Republic
8 Laboratory of Biochemistry and Molecular Genetics Microbial, University of Ouagadougou, 03 P.O. Box: 7131, Ouagadougou 03, Burkina Faso
Annals of Clinical Microbiology and Antimicrobials 2013, 12:14 doi:10.1186/1476-0711-12-14Published: 21 June 2013
Sida urens L. (Malvaceae) is in flora of Asian medicinal herbs and used traditionally in West of Burkina Faso for the treatment of infectious diseases and particularly used against, dental caries bacteria, fever, pain and possesses analgesic properties. This study was conducted to reveal the antibacterial effect against dental caries bacteria on the one hand, and evaluate their analgesic capacity in experimental model with Swiss mice and on the other hand, with an aim to provide a scientific basis for the traditional use of this plant for the management of dental caries bacteria.
The antibacterial assays in this study were performed by using inhibition zone diameters, MIC (Minimum inhibitory concentration) and MBC (Minimal bactericidal concentration) methods. On the whole the dental caries bacteria (Gram-positive and Gram-negative bacterial strains) were used. Negative control was prepared using discs impregnated with 10% DMSO in water and commercially available Gentamicin from Alkom Laboratories LTD was used as positive reference standards for all bacterial strains. In acute toxicity test, mice received doses of extract (acetone/water extract) from Sida urens L. by intraperitoneal route and LD50 was determined in Swiss mice. As for analgesic effects, acetic acid writhing method was used in mice. The acetic acid-induced writhing method was used in mice with aim to study analgesic effects.
The results showed that the highest antibacterial activities were founded with the polyphenol-rich fractions against all bacterial strains compared to the standard antibiotic. About preliminary study in acute toxicity test, LD50 value obtained was more than 5000 mg/kg b.w. Polyphenol-rich fractions produced significant analgesic effects in acetic acid-induced writhing method and in a dose-dependent inhibition was observed.
These results validate the ethno-botanical use of Sida urens L. (Malvaceae) and demonstrate the potential of this herbaceous as a potential antibacterial agent of dental caries that could be effectively used for future health care purposes.